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Image Search Results
Journal: Cancers
Article Title: Honey Targets Ribosome Biogenesis Components to Suppress the Growth of Human Pancreatic Cancer Cells.
doi: 10.3390/cancers16193431
Figure Lengend Snippet: Figure 6. Honey treatment induces apoptosis in PanCa cells. (A). Effect of honey on protein levels of mutant p53, Bcl-2, caspase-3, and PARP in MIA PaCa-2 cells as determined using Western blot analysis after 24 h of honey exposure. Equal loading of protein in each lane was assessed by probing the blots with β-actin antibody. (B). Representative flow cytometry micrographs showing apoptosis in MIA PaCa-2 cells treated with honey for 24 h. The Annexin V/PI-stained cells were analyzed on SONY’ ID7000 Spectral Cell Analyzer. Three independent runs were recorded for each treatment group. (C). The bar graph represents quantification of live, early, and late apoptotic MIA PaCa-2 cells. The data in the bar graph represent the % count ± SEM of three replicates of each dose of honey. Level of significance: ns p = 0.4234; ** p < 0.0027; *** p < 0.0004; **** p < 0.0001. (D). Effect of honey on protein levels of mutant p53, Bcl-2, caspase-3, and PARP in AsPC-1 cells as determined using Western blot analysis after 24 h of honey exposure. Equal loading of protein in each lane was assessed by probing the blots with β-actin antibody. (E). Representative flow cytometry micrographs showing
Article Snippet: The data generated were analyzed using the
Techniques: Mutagenesis, Western Blot, Flow Cytometry, Staining